Carbohydrate Labeling & Analysis Kit

The Carbohydrate Labeling and Analysis Kit is designed for use on the PA 800 Plus and contains the reagents, buffers, and capillaries required to label, separate, and quantify oligosaccharides and monosaccharides released from glycoproteins. After enzymatic or chemical release, sugars are labeled with a fluorophore (APTS) at the reducing termini by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent oligosaccharides are easily resolved in an electric field and detected by laser-induced fluorescence (LIF) detection. This kit provides reagent volumes for analysis of 100 samples and includes: • Carbohydrate Separation Buffer, 56 mL • N-CHO Coated Capillary, 2 units • Labeling Reagents, 1 vial • Labeling Dye (APTS), 4 x 5 mg • Labeling Dye Solvent, 1 mL • Glucose Ladder Standard, 50 mg • Quantitation/Mobility Marker (Maltose), 0.18 mg • APTS-M (monosaccharide-grade), 20 mg