The Fast Glycan Labeling and Analysis kit is designed for use on the PA 800 Plus and is used to rapidly label glycoprotein released N-Glycans with APTS followed by the effective removal of the excess dye using a magnetic bead-based clean-up. The labeled glycans are separated by high-resolution capillary electrophoresis utilizing laser-induced flourescence (LIF) detection and the HR-NCHO separation gel. Glycan identification is determined based on their normalized electrophoretic mobility referenced against pre-determined GU values using the appropriate bracketing standard.
This kit provides reagent volumes for analysis of 100 samples and includes:
• M1, 22 mL
• D1, 0.05 mL x 5 vials
• D2, 250mM/50 µL dried x 5 vials
• D3, 1.5 mL x 1 vial
• D4, 1.5 mL x 2 vials
• L5, 5 mg x 1 vial
• L6, 5 mg x 5 vials
• GU Ladder, 5 mg x 1 vial
• IST (Internal Standard), 5 mg x 1 vial
• BST (Bracketing Standard), 10 pmol x 1 vial
• HR-NCHO Separation Gel, 56ml
• Magnetic Separator, 1 unit
• Pre-Assembled Cartridge (P/N A55625), 1 unit
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