CE-SDS Protein Analysis Kit

The CE-SDS Protein Analysis Kit is configured for use on the BioPhase 8800 system. This kit utilizes a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. Additionally, this assay can assess the purity and heterogeneity of IgG reagents in both a reduced and non-reduced state. The methodology involves heat denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS and separating these proteins by size using high-resolution capillary gel electrophoresis technology. This assay will detect impurities as low as 0.01% (utilizing laser-induced fluorescence) and can be utilized for either size or purity/heterogeneity analysis depending on the standards used. This kit enables 200 tests. This kit includes: • SDS Gel Separation Buffer • SDS Sample Buffer • Low pH SDS Sample Buffer • Internal Standard, 10 kDa protein, 5 mg/mL • Acidic Wash Solution, 0.1 N HCl • Basic Wash Solution, 0.1 N NaOH • CE Grade Water

$ 2,725.00

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