IgG Purity/ Heterogeneity Assay Kit

This kit allows for the assessment of purity and heterogeneity of proteins in both a reduced and non-reduced state. The methodology involves heat denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS and separating these proteins by size using high-resolution capillary gel electrophoresis technology. This assay will detect impurities as low as 0.1% (0.01% if Laser-Induced Fluoresence (LIF) detection and dye are used) and includes an IgG protein control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns. The kit includes Bare-Fused Silica Capillary, 50 µm ID x 57 cm (Qty: 2); SDS-MW Gel Buffer (Qty: 140 mL); SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS (Qty: 50 mL); IgG Control Standard, 1 mg/mL in SDS sample buffer (Qty: 1 mL); Internal Standard, 10 kDa protein, 5 mg/mL (Qty: 0.4 mL); Acidic Wash Solution, 0.1 N HCl (Qty: 100 mL); and Basic Wash Solution, 0.1 N NaOH (Qty: 100 mL).



A10663
$ 1,087.21
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