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Short DescriptioncIEF Kit
Long DescriptionThe cIEF Kit configured for use on the BioPhase 8800 system. This kit will accurately determine a protein’s charge heterogeneity, which helps establish identity and stability. Capillary Isoelectric Focusing (cIEF) is a powerful technique that allows quantitative analysis of a protein’s isoelectric point (pI). In cIEF, a mixture of sample and ampholyte is introduced into a capillary and subjected to electrophoretic separation. In this process, a pH gradient through which analytes migrate to their respective pI is formed. Advanced cIEF technology is necessary to successfully achieve high precision and quantitative separations. Use of optimized universal methods and synthetic pI markers attain the highest levels of precision in pI calculation and direct isoform quantitation with UV detection. This kit enables 200 tests. This kit includes: • Cathodic Stabilizer • Anodic Stabilizer • cIEF Gel • Urea • Neutral Capillary Conditioning Solution • Anolyte • Catholyte • Chemical Mobilizer • Formamide • CE Grade Water NOTE: Additional reagents from other vendors are required for this application. Methods and necessary supplies can be referenced in the application guides found on
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